한빛사 논문
Sunho Park,1 John D. Karalis,2 Changjin Hong,1 Jean R. Clemenceau,1 Matthew R. Porembka,2 In-Ho Kim,3 Sung Hak Lee,4 Sam C. Wang,2* Jae-Ho Cheong,5,6,7* Tae Hyun Hwang1,8,9*
1 Department of Artificial Intelligence and Informatics, Mayo Clinic, Jacksonville, FL
2 Division of Surgical Oncology, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX
3 Department of Internal Medicine, Division of Medical Oncology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea
4 Department of Hospital Pathology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea
5 Department of Surgery and
6 Department of Biochemistry and Molecular Biology,
7 Department of Biomedical Systems Informatics, Yonsei University College of Medicine, Seoul, South Korea
8 Department of Immunology, Mayo Clinic, Jacksonville, FL
9 Department of Cancer Biology, Mayo Clinic, Jacksonville, FL
Sunho Park and John D. Karalis contributed equally as co-first authors.
Sam C. Wang, Jae-Ho Cheong, and Tae Hyun Hwang are co-corresponding authors.
*Corresponding authors: Sam C. Wang MD, Jae-Ho Cheong MD PhD, Tae Hyun Hwang PhD
Abstract
Purpose: We sought to identify biomarkers that predict overall survival and response to immune checkpoint inhibitors (ICI) for gastric cancer patients.
Experimental design: This was a retrospective study of multiple independent cohorts of gastric cancer patients. The association between tumor ACTA2 expression and overall survival and ICI response were determined in patients whose tumors were analyzed with bulk mRNA sequencing. Single cell RNA-sequencing and digital spatial profiling data were used to compare tumors from gastric cancer patients who did and did not respond to ICI.
Results: Increasing tumor ACTA2 expression was independently associated with worse overall survival in a 567-patient discovery cohort (HR: 1.28 per unit increase, 95%CI: 1.02-1.62). This finding was validated in three independent cohorts (n=974; HR: 1.52 per unit increase, 95%CI: 1.34-1.73). Of the 108 patients treated with ICI, 56% of patients with low ACTA2 expression responded to ICI versus 25% of patients with high ACTA2 expression (p=0.004). In an analysis of a publicly available single cell RNA-sequencing dataset of 5 microsatellite instability-high patients treated with ICI, the patient who responded to ICI had lower tumor stromal ACTA2 expression than the 4 non-responders. Digital spatial profiling of tumor samples from 4 ICI responders and 5 ICI non-responders revealed that responders may have lower ACTA2 expression in α-SMA-positive cancer-associated fibroblasts than non-responders (median: 5.00 vs. 5.50).
Conclusions: ACTA2 expression is associated with survival and ICI response in gastric cancer patients. ACTA2 expression in cancer-associated fibroblasts, but not in other cellular compartments, appears to be associated with ICI response
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