한빛사 논문
Sungmi Choi PhD1,2, Kyoung-Hee Sohn MD, PhD3,4, Jae-Woo Jung MD, PhD5, Min-Gyu Kang MD, PhD6, Min-Suk Yang MD, PhD7, Sujeong Kim MD8, Jeong-Hee Choi MD, PhD9,10, Sang-Heon Cho MD, PhD3,11, Hye-Ryun Kang MD, PhD3,11,*, Hana Yi PhD1,2,12,*
1Institute for Biomaterials, Korea University, Seoul, Korea
2Interdisciplinary Program in Precision Public Health, Korea University, Seoul, Korea
3Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Korea
4Department of Internal Medicine, Kyung Hee University Hospital, Seoul, Korea
5Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea
6Department of Internal Medicine, Chungbuk National University College of Medicine, Chungbuk National University Hospital, Cheongju, Korea
7Department of Internal Medicine, SMG-SNU Boramae Medical Center, Seoul, Korea
8Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Korea
9Department of Pulmonology and Allergy, Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Korea
10Allergy and Clinical Immunology Research Center, Hallym University College of Medicine, Chuncheon, Korea
11Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea
12School of Biosystems and Biomedical Sciences, Korea University, Seoul, Korea
*Corresponding author
Abstract
Background
Although some respiratory virus infections are known to contribute to the development and exacerbation of asthma, commensal virome in airway have not been extensively studied due to technical challenges.
Objectives
We investigated the characteristics of the virome in asthmatic airways.
Methods
Both the bacteriome and virome profiles in sputum from 12 healthy individuals, 15 patients with non-severe asthma, and 15 patients with severe asthma were analyzed and assessed for the association with clinical characteristics such as severity, exacerbation, Asthma Control Test (ACT), and lung function.
Results
While analysis of the 16S rRNA bacteriome in the airway showed no differences, clear contrasts in the diversity and composition of airway virome were observed between healthy controls and patients with asthma. Herpesviruses were the most abundant type of virus in the asthma group (44.6% ± 4.6%), mainly with cytomegalovirus (CMV) and Epstein-Barr virus (EBV) accounting for 24.5% ± 3.3% and 16.9% ± 3.5% respectively in contrast to those in the healthy controls (5.4% ± 2.5% and 7.1% ± 3.0%, respectively). CMV and EBV were more abundant in asthmatics who experienced exacerbation and their abundance showed correlation with more severe asthma, lower ACT score, and lower lung function. On the contrary, bacteriophage abundant in healthy controls was severely reduced in asthma patients in the order of non-severe and severe asthma and presented significant positive correlation with ACT and FEV1/FVC.
Conclusion
Lung virome, especially, CMV, EBV, and bacteriophage may be potential biomarkers of asthma severity and exacerbation.
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