한빛사 논문
Sangrak Jina,b, Yale Jeonc, Min Soo Jeonc, Jongoh Shina,b, Yoseb Songa,b, Seulgi Kanga,b, Jiyun Baea,b, Suhyung Choa,b, Jung-Kul Leed, Dong Rip Kimc, and Byung-Kwan Choa,b,e,1
aDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, 34141 Daejeon, Republic of Korea; bInnovative Biomaterials Research Center, KI for the BioCentury, Korea Advanced Institute of Science and Technology, 34141 Daejeon, Republic of Korea; cDepartment of Mechanical Engineering, Hanyang University, 04763 Seoul, Republic of Korea; dDepartment of Chemical Engineering, Konkuk University, 05029 Seoul, Republic of Korea; and eIntelligent Synthetic Biology Center, 34141 Daejeon, Republic of Korea
1To whom correspondence may be addressed.
Abstract
Acetogenic bacteria use cellular redox energy to convert CO2 to acetate using the Wood–Ljungdahl (WL) pathway. Such redox energy can be derived from electrons generated from H2 as well as from inorganic materials, such as photoresponsive semiconductors. We have developed a nanoparticle-microbe hybrid system in which chemically synthesized cadmium sulfide nanoparticles (CdS-NPs) are displayed on the cell surface of the industrial acetogen Clostridium autoethanogenum. The hybrid system converts CO2 into acetate without the need for additional energy sources, such as H2, and uses only light-induced electrons from CdS-NPs. To elucidate the underlying mechanism by which C. autoethanogenum uses electrons generated from external energy sources to reduce CO2, we performed transcriptional analysis. Our results indicate that genes encoding the metal ion or flavin-binding proteins were highly up-regulated under CdS-driven autotrophic conditions along with the activation of genes associated with the WL pathway and energy conservation system. Furthermore, the addition of these cofactors increased the CO2 fixation rate under light-exposure conditions. Our results demonstrate the potential to improve the efficiency of artificial photosynthesis systems based on acetogenic bacteria integrated with photoresponsive nanoparticles.
acetogenic bacteria, artificial photosynthesis, cadmium sulfide nanoparticle, extracellular electron transfer, Clostridium autoethanogenum
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