한빛사 논문
성균관대학교, Brigham and Women’s Hospital, Harvard Medical School, University of California at Berkeley
Yuchao Chen1,2,9, Qingfu Zhu1,9, Liming Cheng3, Yong Wang2, Meng Li4, Qinsi Yang2, Liang Hu1, Doudou Lou1, Jiaoyuan Li3, Xianjun Dong5, Luke P. Lee 6,7,8,* and Fei Liu1,2,*
1Eye Hospital, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, China. 2Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, China. 3Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 4College of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an, China. 5Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA. 6Renal Division and Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA. 7Department of Bioengineering, Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA, USA. 8Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Korea. 9These authors contributed equally: Yuchao Chen, Qingfu Zhu.
*Correspondence to Luke P. Lee or Fei Liu.
Abstract
Exosomes have shown great potential in disease diagnostics and therapeutics. However, current isolation approaches are burdensome and suffer from low speed, yield and purity, limiting basic research and clinical applications. Here, we describe an efficient exosome detection method via the ultrafast-isolation system (EXODUS) that allows automated label-free purification of exosomes from varied biofluids. We obtained the ultra-efficient purification of exosomes by negative pressure oscillation and double coupled harmonic oscillator–enabled membrane vibration. Our two coupled oscillators generate dual-frequency transverse waves on the membranes, enabling EXODUS to outperform other isolation techniques in speed, purity and yield. We demonstrated EXODUS by purifying exosomes from urine samples of 113 patients and validated the practical relevance in exosomal RNA profiling with the high-resolution capability and high-throughput analysis.
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