한빛사 논문
Sathya Balachander1,7, Alli L. Gombolay1,7, Taehwan Yang1,7, Penghao Xu1,7, Gary Newnam1 , Havva Keskin1,5, Waleed M. M. El-Sayed1,6, Anton V. Bryksin2, Sijia Tao3, Nicole E. Bowen3, Raymond F. Schinazi3, Baek Kim3, Kyung Duk Koh4, Fredrik O. Vannberg1 & Francesca Storici1,*
1School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
2Molecular Evolution Core, Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.
3Department of Pediatrics, School of Medicine, Emory University, Atlanta, GA, USA.
4Lung Biology Center, Department of Medicine, University of California San Francisco, San Francisco, CA, USA.
5Present address: Omega Bio-tek, Norcross, GA, USA.
6Present address: Marine Microbiology Department, National Institute of Oceanography and Fisheries, Red Sea, Egypt.
7These authors contributed equally: Sathya Balachander, Alli L. Gombolay, Taehwan Yang, Penghao Xu.
*Corresponding author
Abstract
Despite the abundance of ribonucleoside monophosphates (rNMPs) in DNA, sites of rNMP incorporation remain poorly characterized. Here, by using ribose-seq and Ribose-Map techniques, we built and analyzed high-throughput sequencing libraries of rNMPs derived from mitochondrial and nuclear DNA of budding and fission yeast. We reveal both common and unique features of rNMP sites among yeast species and strains, and between wild type and different ribonuclease H-mutant genotypes. We demonstrate that the rNMPs are not randomly incorporated in DNA. We highlight signatures and patterns of rNMPs, including sites within trinucleotide-repeat tracts. Our results uncover that the deoxyribonucleotide immediately upstream of the rNMPs has a strong influence on rNMP distribution, suggesting a mechanism of rNMP accommodation by DNA polymerases as a driving force of rNMP incorporation. Consistently, we find deoxyadenosine upstream from the most abundant genomic rCMPs and rGMPs. This study establishes a framework to better understand mechanisms of rNMP incorporation in DNA.
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