한빛사 논문
Myung-Hee Kim1, Jieun Jeon1,2, Seulbee Lee1, Jae Ho Lee1,2, Lei Gao3, Byung-Hoon Lee2, Jeong Mee Park4, Yun Ju Kim1,* and June M. Kwak2,*
1 Center for Plant Aging Research, Institute for Basic Science, Daegu, Republic of Korea.
2 Department of New Biology, DGIST, Daegu, Republic of Korea.
3 Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
4 Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
*Correspondence to Yun Ju Kim or June M. Kwak
Abstract
RNA quality control (RQC) and post-transcriptional gene silencing (PTGS) target and degrade aberrant endogenous RNAs and foreign RNAs, contributing to homeostasis of cellular RNAs. In plants, RQC and PTGS compete for foreign and selected endogenous RNAs; however, little is known about the mechanism interconnecting the two pathways. Using a reporter system designed for monitoring PTGS, we revealed that the 26S proteasome subunit RPT2a enhances transgene PTGS by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis. RPT2a physically associated with a subset of RQC components and downregulated the protein level. Overexpression of the RQC components interfered with transgene silencing, and impairment of the RQC machinery reinforced transgene PTGS attenuated by rpt2a. Overall, we demonstrate that the 26S proteasome subunit RPT2a promotes PTGS by repressing the RQC machinery to control foreign RNAs.
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