한빛사 논문
Sang-Chul Kima,b, Dmitri A. Nusinowb, Maria L. Sorkinb,c, Jose Pruneda-Pazd, Xuemin Wanga,b,*
a Department of Biology, University of Missouri, St. Louis, MO 63121, USA
b Donald Danforth Plant Science Center, St. Louis, MO 63132, USA
c Division of Biology and Biomedical Sciences, Washington University in St. Louis, St. Louis, MO 63110, USA
d 13 Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
*Corresponding author : Xuemin Wang
Abstract
Circadian clocks play important roles in regulating cellular metabolism, but the reciprocal effect that metabolism has on the clock is largely unknown in plants. Here we show that the central glycerolipid metabolite and lipid mediator phosphatidic acid (PA) interacts with and modulates the function of the core clock regulators LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) in Arabidopsis. PA reduced the ability of LHY and CCA1 to bind the promoter of their target gene TIMING OF CAB EXPRESSION1. Increased PA accumulation and inhibition of PA-producing enzymes had opposite effects on circadian clock outputs. Diurnal change in levels of several membrane phospholipid species, including PA, observed in wild-type was lost in the LHY and CCA1 double knockout mutant. Storage lipid accumulation was also affected in the clock mutants. These results indicate that the interaction of PA with the clock regulator may function as a cellular conduit to integrate the circadian clock with lipid metabolism.
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