[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보 회원가입   로그인
BRIC홈 Bio마켓
올림푸스한국
배너광고안내
이전
다음
스폰서배너광고 안내  배너1 배너2 배너3 배너4
브릭이만난사람들
전체보기 할인행사 시약벤더 제품평가 기업정보 BRIC인증마크
 
조회 143  인쇄하기 주소복사 트위터 공유 페이스북 공유 
[바이오클론] [APExBIO한국공식대리점] 3X (DYKDDDDK) Peptide
제품(기술)분류: 시약 > Chemical Reagent
전화: 02-2690-0058
메일: bioclone(at).bioclone.co.kr
팩스: 02-2690-0397

확대보기
상세정보 사용후기 회사정보 |  등록제품 |  시약벤더 |  진행중 이벤트
  카탈로그 NO :
  제품 생산일 :
  원산지 :
  생산기업 :
  특징 및 규격  

 

APExBIO는 Small molecule, Inhibitor, Peptide 등의

chemical 관련 제품을 직접 생산, 공급하는 브랜드입니다.
 
 

바이오클론(주)는 

APExBIO의 한국공식대리점이며, 

2015년부터 정부의 화학물질의 등록 및 평가 등에

관한 법률(화평법)에 의해 모든 화학물질을 한국환경공단에

신고, 허가 하에 안전하게 수입 공급하고 있습니다.

한국환경공단 신고허가없이 수입되거나 공식대리점이 아닌 무분별한 수입업자에 공급되어진 화학물질품목은 화평법에 의해 문제소지가 발생할수 있습니다

Background

The DYKDDDDK-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1.

The DYKDDDDK peptide binds to the antibody M1. Whether binding is calcium-dependent manner2 or –independent3 remains controversial.

A disadvantage of the system is that the monoclonalantibody purification matrix is not as stable as others. In general, small tags can be detected with specific monoclonal antibodies.

To improve the detection of the DYKDDDDK tag the 3x (DYKDDDDK) system has been developed. This threetandem DYKDDDDK epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein.

The DYKDDDDK-tagged maltodextrin-binding protein of Pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.

Finally, the DYKDDDDK-tag can be removed by treatment with enterokinase, which is specific for the five C-terminal amino acids of the peptide sequence5.

 

References:


1. Hopp TP, Prickett KS, Price VL, Libby RT, March CJ, Ceretti DP, Urdal DL, Conlon PJ (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6:1204–1210.


2. Hopp TP, Gallis B, Prikett KS (1996) Metal-binding properties of a calcium dependent monoclonal antibody. Mol Immunol 33:601–608.


3. Einhauer A, Jungbauer A (2000) Kinetics and thermodynamical properties of the monoclonal antibody M1 directed against the FLAG peptide. 20th International symposium on the separation of proteins, peptides, and polynucleotides (ISPPP). Lublijana, Slovenia, November 5–8, 2000.


4. Bucher MH, Evdokimov AG, Waugh DS (2002) Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Biol Cryst 58:392–397.


5. Maroux S, Baratti J, Desnuelle P (1971) Purification and specificity of procine enterokinase. J Biol Chem 246:5031–5039.

 

 

Chemical Properties

Storage Desiccate at -20°C
M.Wt 2861.87
Formula C120H169N31O49S
Synonyms H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
Solubility ≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl), 1X TBS as recommended buffer.
Chemical Name 3X (DYKDDDDK) Peptide
Canonical SMILES

CCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCC

CN)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC2=CNC=N2)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C(CC(=O)O)NC(

Shipping Condition Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tips For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

 

 Protocol 

ELISA experiment [1]:

Preparation method

The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80°C for several months.

Applications

3-DYKDDDDK peptide has found widespread use as a mild purification reagent for DYKDDDDK-epitope tagged recombinant proteins.

Although its affinity columns release monovalent flagged proteins in the absence of calcium, the antibody retains substantial affinity for the DYKDDDDK sequence even in metal-free conditions, so that it has been impossible to use it to develop a metal-sensitive ELISA assay.

This is due to the ability of the antibody to remain bound to polyvalent surface-coated antigen, for instance, when Flagged proteins are bound to ELISA plates or blotting filters. The resultant antigen polyvalence raises the avidity of the DYKDDDDK antibody to a point where the reaction is essentially calcium-independent.

However, when the antibody itself was made monovalent, by proteolytic cleavage to the Fab, this situation was reversed and the ELISA reaction became calcium-dependent. This new metal-dependent ELISA assay was used to explore the metal requirements of the antibody in detail. Among divalent metals, binding tapered off with increasing radius above that of calcium, or with decreasing radius below that of calcium.

Several smaller metals, such as nickel, acted as inhibitors of the binding reaction. Substantial binding was demonstrated for heavy metals such as cadmium, lanthanum and samarium. Because it is of interest to use this antibody for the co-crystallization of recombinant DYKDDDDK-fusion proteins, the ability to bind heavy metals was a significant finding.

 

매주 금요일 4시 발주 1-2 주 소요

자세한 문의사항은 바이오클론으로 연락주세요

Homepage : www.bioclone.co.kr

 

upload_image

 

 한국공식대리점 바이오클론㈜

T) 02-2690-0058 F) 02-2690-0397

www.bioclone.co.kr

 

사용후기
작성자*
내용*
만족도*
할인행사 광고 할인행사 광고
필코리아테크놀로지
에펜도르프코리아 두둥! 최대 원쁠원 행사부터 20% 행사까지! 없는거 빼고 다 있는 필코리아테크놀로지 할인...
필코리아테크놀로지
에펜도르프코리아 우체통 열어주세요 필코리아테크놀로지의 뉴스레터가 들어가니까요!!
유어랩
에펜도르프코리아 [마지막 한달] ABclonal ELISA kit 최대 55% 할인 기회를 잡으세요
바이오닉스
에펜도르프코리아 [TECAN공식대리점] 봄맞이 사은이벤트
그린메이트바이오텍
에펜도르프코리아 [IMPLEN공식대리점] UV/Vis Spectrophotometer 구매 시 선착순 멀티...
그린메이트바이오텍
에펜도르프코리아 [TECAN한국대리점 20주년] Microplate reader 초특가 이벤트!! 20년...
브래디
에펜도르프코리아 초저온라벨 등 실험실용 라벨링 솔루션
위로가기
Bio마켓 홈  |  Bio마켓FAQ  |  Bio마켓 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
머크 광고