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[RJH 한국독점대리점] All-Fect: Transfection Reagent
바이오클론
RJH BIOSCIENCES는
In Vitro/In Vivo Transfection Reagents
전문 기업입니다.
https://www.rjhbiosciences.com/
"RJH Biosciences is a biotechnology company
whose focus is to realize the full potential
of nucleic acids for human health.
We develop novel transfection reagents and delivery systems that transport different types of
nucleic acids to a range of human cells."
All-Fect is a highly effective, broadly-acting gene delivery system useful in suspension cell transfection and It has been tailored as a siRNA pDNA cotransfection reagent for a variety of cell lines and is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Al-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. All-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent making it an excellent choice for transient transfection applications. All-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension cells. As with all transfection reagents, formulations of All-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.
pDNA delivery of GFP plasmid to cord-blood derived mesenchymal stem cells with Lipofectamine and All-Fect
Transfecting attachment-independent K562 cells with plasmid DNA and siRNA using ALL-Fect and a leading lipofection reagent. (Left) GFP expression was induced with plasmid DNA and analyzed by flow cytometry 2 days after transfection. (Right) Survivin silencing and resultant inhibition of cell growth with specific siRNA 3 days after transfection.
Transfecting attachment-dependent MDA-MB-231 cells with plasmid DNA and siRNA using ALL-Fect and a leading lipofection reagent. (Left) GFP expression was induced with plasmid DNA and analyzed by fluorescent microscopy (insert) and flow cytometry 2 days after transfection. (Right) GFP silencing was induced with siRNA and analyzed by flow cytometry 2 days after transfection.
Benefits of RJH Transfection Reagents
• 특정 셀 유형에 맞게 맞춤화 된 고효율의 transfection
• Automation 및 scale-up을 위한 간편한 protocol
• Target cells의 독성을 최소화하여 complication 없이
nucleic acid 효과들을 나타낼 수 있습니다.
• 효율적인 비용의 reagent로 인해 large screen에서
추가 비용이 최소화됩니다.
• Animal model에서 동일한 transfection reagent를
사용할 수 있으므로 일관된 연구가 가능합니다.
자세한 문의사항은 본사로 문의해 주시기 바랍니다.
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