연구용제품시약 > Antibody
Ion Channels | News & Updates
엘바이오시스템
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Products for Immuno-Colocalization
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Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:
Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.
Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IHC and ICC.
Toxins conjugated to ATTO-fluorescent dyes: These labeled toxins can be used with any antibody to obtain highly specific channel localization.
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Anti-Presenilin-1-ATTO-488 Antibody (#AIP-011-AG) Immuno-colocalization of Calnexin and Presenilin-1 in mouse cortex:
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Products for Live Cell Flow Cytometry
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We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.
With Alomone Labs extracellular antibodies:
No need for cell permeabilization and fixation
Cell surface detection of proteins
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Products for Live Cell Imaging (LCI)
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Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.
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Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027) Cell surface detection of NaV1.7 in intact living rat pheochromocytoma PC12 cells:
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Labeled Toxins as Probes for Detecting Ion Channels
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Studying membrane proteins, namely ion channels, remains a challenge due to the nature of their complex structure. Using the unique and exclusive properties of peptide toxins, we have developed a novel tool for detecting ion channels in living cells and tissues.
Benefits of using labeled toxins:
Localization and distribution
Clustering and internalization kinetics
Live cell imaging
Single cell detection
Binding kinetics
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A Bit of Color Goes a Long Way
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Alomone Labs MmTx1 Toxin-ATTO-488 labels GABA(A) α1 receptor in rat cerebellum:
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Alomone Labs MmTx1 Toxin ATTO-488 binds to rat cerebellum:
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Ion Channel Products in Featured Papers
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A Potential Role for Kir5.1 Channel in pH Sensitivity in 5-HT Neurons Serotonin (5-HT) neurons in the brain stem play an important role in maintaining/modulating ventilation and pH sensitivity for proper functioning of the neural network. A search of age-related changes of pH-sensitive molecules in 5-HT neurons gave rise to the identification of Kir5.1 channel as a potential candidate in CO2/pH chemosensitivity in brainstem 5-HT neurons. Immunohistochemical staining of rat brainstem sections using Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) and Anti-Kir5.1 Antibody (#APC-123) showed that both channels are expressed (green). However, Kir4.1 staining is limited to astrocytes, while that of Kir5.1 is detected in 5-HT neurons (Figure 1). The authors do not rule out the possibility that other proteins, namely pH-sensitive ion channels may also be involved.
Figure 1. Expression of Kir4.1 and Kir5.1 channels in rat brainstem:
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Adapted from Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34. with permission of Frontiers.
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