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Advanced BioMatrix
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Stiffness of Collagen 3D Hydrogels A White Paper by Advanced BioMatrix | ||
The stiffness of 3D hydrogels in cell culture can impact a wide variety of pathways including stem cell differentiation, cancer metastasis, and cardiac tissue function. Our team at Advanced BioMatrix has released a white paper compiling gel stiffness data of our 5 most popular type I collagen products for 3D hydrogels. Each of these products is manufactured under stringent controls at our facility. Each lot undergoes >10 quality control tests prior to release, including gel stiffness rheology. The collagen products that we tested for this white paper include collagen concentrations ranging from 3-10 mg/ml, and are either enzyme extracted (atelocollagen) or acid extracted (telocollagen). | ||
Gel stiffness testing included collecting data points across three commonly used rheological methods: -Atomic force microscopy -Parallel plate dynamic shear rheometry (Bohlin) -- (see graph below) -Contactless measurement system (ElastoSens) | ||
The table above shows the plateau storage modulus (G') taken from the parallel plate rheometer. The two main conclusions from this data show that increasing the collagen concentration will significantly increase gel stiffness, and acid extracted collagen hydrogels are significantly firmer than enzyme extracted collagen hydrogels at similar concentrations. The full paper can be read on our website by clicking on any of the above images, or by clicking here. Read the full paper to see all of our data, methods, parameters, considerations, conclusions and discussion points on substrate stiffness with 3D collagen hydrogels. | ||
Please visit our website for our full product listing of extracellular matrix products for 2D coatings, 3D hydrogels and 3D bioprinting. -The Advanced BioMatrix team |
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