High Performance- Provide up to eight-fold higher functional titers
Simple Protocol- No media change required, single harvest
Animal Origin Free -Regulatory friendly
그림 1. Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 2 μg/well) with the following reagents:TransIT®-Lenti (3:1, vol:wt), Lipofectamine™ 2000 (3:1), Lipofectamine™ 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 μg pDNA/well). The supernatant was harvested, filtered (0.45 μm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 μg/mlTransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.