Another area of growing interest in clinical research is the discovery and validation of potential biomarkers that may be used individually or together to signal disease onset or progression, response to therapy, drug toxicity and other clinical conditions. LC/MS/MS is ideally suited to identify panels of steroids, peptides, vitamins, and other compounds in complex samples such as blood, urine, saliva and whole cell lysates.
For example, steroid analysis by conventional immunoassay methods lacks specificity and only detects different steroids one at a time. With LC/MS/MS, you can analyze an entire panel of steroids simultaneously, with high sensitivity and specificity.
Simultaneous analysis of a panel of 10 steroids (IS: Internal Standard).
iMethod™ Test for Vitamin D Version 1.0 for Cliquid® Software
Scientific poster: Routine Use of a Methodology for Dosing 9 Steroids in Clinical Research Samples by LC/MS/MS (0.2 MB PDF)