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[에이비사이엑스코리아] SimGlycan™ Software for Glycan Analysis
제품(기술)분류: 소프트웨어 > Genomes
전화: 02-575-5660
메일: sanghwa.kim(at).absciex.com
팩스: 02-575-5664

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SimGlycan™ Software

SimGlycan™ Software (a product of PREMIER Biosoft International) predicts the structure of a glycan from the MS/MS data acquired by AB SCIEX mass spectrometers. It facilitates the study of glycosylation and post translational modifications. The software works directly with *.t2d and *.wiff data generated from AB SCIEX instruments, including the 4800 Plus MALDI TOF/TOF™ Analyzer, 4000 QTRAP® System, and QSTAR® Elite System. A one year license is included. Thereafter, for continued product use, this license will have to be renewed for each successive one-year term by paying the prevailing Annual License Fee, available from the Premier Biosoft Web Site

Product Description

SimGlycan™ Software (a product of PREMIER Biosoft International) predicts the structure of a glycan from the MS/MS data acquired by AB SCIEX mass spectrometers. It facilitates the study of glycosylation and post translational modifications. The software works directly with *.t2d and *.wiff data generated from AB SCIEX instruments, including the 4800 Plus MALDI TOF/TOF™ Analyzer, 4000 QTRAP® System, and QSTAR® Elite System.

  • Matches glycan MS/MS data with its own database of theoretical fragmentation spectra of over 8,000 glycans by using a proprietary ranking and scoring algorithm.
  • Database can be searched using ID, sequence, composition or mass.
  • References other biological information for the probable glycan structures such as glycan class, reaction, pathway and enzyme.
  • You can perform structural analysis of glycopeptides by specifying the sequence or mass of the attached peptide moiety.
  • Imports experimental MS/MS data generated by all AB SCIEX mass spectrometers enabling you to launch it directly from Analyst® QS 2.0 and Analyst 1.5 Software using the companion software feature.
  • Enables you to search for glycans with chemical derivatives used for reducing end modifications even if the derivative is not available in the program's database.

    cms_055177.jpg (1060×752)

    Takes Advantage of the Unique Capabilities of AB SCIEX InstrumentsBecause of its large mass range, high sensitivity, and soft desorption and ionization, MALDI time-of-flight (MALDI-TOF) mass spectrometry is widely used in the molecular weight determination of underivatized oligo- and polysaccharides. Traditionally, post source decay (PSD) has been used for structural analysis, and this can provide information related to sequence and branching, however, a lack of abundant cross-ring cleavages limits the linkage information that can be deduced from such experiments.

    The 4800 Plus MALDI TOF/TOF™ Analyzer is the only instrument that combines high energy MS/MS for cross ring fragmentation for carbohydrate analysis (A and X- ions) with good precursor ion selection. This permits acquisition of more information on linkage position and points of branching, allowing the automated search routine in SimGlycan Software to correctly identify and characterize glycans without any secondary experiments. 

    QTRAP® systems are unique hybrid triple quadrupole linear ion trap instruments where a triple quadrupole scan function (precursor ion scan, MRM) can be coupled with a high sensitivity ion trap MS/MS and MS3 scan to enable sensitive and selective experiments for glycan structure and connectivity. Because of the unique instrument configuration in the QTRAP system, the MS/MS spectra do not show the low mass cut-off experienced by 3D-ion traps. Subsequent MS3 fragmentation spectra can be obtained allowing mechanistic studies to be performed on the diagnostic fragments to confirm a predictive SimGlycan search result.
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