The CYP450 Protein Assay – Human Induction Kit, uses an LC/MS workflow to enable direct measurement of protein expression changes of individual CYP450 isoforms with high specificity, sensitivity, and accuracy. The kit has been developed to employ MRM analysis to quantify the protein levels of four key CYP450 protein isoforms for induction studies: 1A2, 2B6, 3A4, and 3A5.
Directly measure CYP450 induction at the protein level without expensive antibodies
Measure individual CYP450 isoforms with high specificity, sensitivity, and accuracy
Easy-to-use kits and method employ a common MRM workflow
Results are equivalent to traditional mRNA or activity assays
Everything You Need to Perform Protein QuantitationThe Starter kit contains peptide standards, sample preparation reagents and buffers, trypsin, a column, data acquisition methods, and processing methods and templates for performing protein quantitation using MRM analysis on an AB SCIEX Triple Quad™ or QTRAP® system. The peptide standards for each protein are synthesized using isotopically enriched amino acids (lysine and arginine) to provide the highest quantitative robustness.