ABI PRISM? 7000 Sequence Detection System은 Nucleic acid sequences를 검출 및 정량 하는 경제적인 real-time PCR system입니다.
1. PCR system과 형광 검출 장치가 통합되어 PCR products의 실시간 정량 가능
2. Peltier-based, 96-well block thermal cycling system
3. Scheme: Tungsten-halogen lamp에 의해excitation된 sample의 형광은, 4개의 optical filters에 의해 filtering이 된 후cooled charge-coupled device (CCD) camera에 capture됨
4. 4개의 emission filters는 FAM™/SYBR? Green 1, VIC™/JOE, NED™/TAMRA™, ROX™ dyes에 optimization되도록 고안되어 있음
5. Multicolor detection 가능
6. Notebook이 제공되는 small footprint의 기기
7. Primer Express? Software 내장
8. Chemistry: TaqMan Chemistry & SYBR Green Chemistry
9. Application: PCR quantitation, Disease screening, detection of pathogens, Allele discrimination, Viral detection, Mutation screening, Plus/ minus assay
- ABI PRISM? 7000 Sequence Detection System:
Peltier-based, 96-well block thermal cycling system
Tungsten-halogen lamp excitation source
Fluorescence detection via a four-position filter wheel and CCD camera
The software runs on Windows? 2000 operating system and is used for instrument control, data collection, and data analysis. Real-time monitoring during data collection. Intuitive multiplex assay set-up and analysis. Simple dissociation curve data collection and viewing. Intuitive allelic discrimination results viewer. Automatic identification of samples containing a PCR inhibitor when performing plus/minus assays with an IPC.
- Performance: Using the TaqMan RNase P Instrument Verification Plate, the ABI PRISM? 7000 Sequence Detection System can distinguish between 5,000 and 10,000 template copies with 99.7% confidence.