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Thermo Dharmacon RNAi Total Solution! - ㈜서린
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Thermo Scientific Dharmacon Product Overview
- Superior RNAi products and services for high-confidence results
- Scientific innovation empowering siRNA potency, specificity and delivery
- Potent microRNA products from the RNAi experts
Thermo Scientific Dharmacon siRNA Reagents
Accell siRNA Reagents
RNAi research no longer is constrained by cell types that are resistant to conventional lipid-based delivery reagents. Thermo Scientific Dharmacon Accell siRNA provides delivery into any cell type for unprecedented experimental flexibility and discovery.
A Neuroblastoma cells (SH-SY5Y; panel A) and T-lymphocyte cells (Jurkat; panel B) were treated with 1 μM Accell Red Non-targeting control siRNA (Cat # D-001960-01) and 1 μM Accell human GAPDH Control pool (Cat # D-001930-10) in Accell delivery media (Cat# B-005000) for 72 hours. Live cells were imaged by epifluorescence to demonstrate effective cellular uptake of the labeled control (Red; cytoplasm = Accell NTC Red and Blue; nucleus = Hoechst). B GAPDH knockdown was assessed by ABgene Verso SYBR Green qRT-PCR Kit. The average expression levels of three biological replicates is shown. Beta-Actin was used as a housekeeping gene for normalization. Expression levels were further normalized to NTC. |
Successful cell types where Accell siRNA has delivered effective knockdown
Human Suspension Cells | Jurkat • THP-1 • IM-9 • BDCM |
Human Differentiated Stem Cells | Osteoblasts and adipocytes derived from hMSC |
Human, Mouse, and Rat Primary Cells | HUVEC • HUASMC • hMSC • PBMC • Primary human astrocytes • Primary human T cells • Primary human keratinocytes • Primary mouse hepatocytes • Primary rat cortical neurons • Primary rat striatum neurons |
Other Mouse and Rat Cells | 3T3 NIH • ES-D3 • 3T3 L1 • Rat2 • C2C12 • H9c2 • PC-12 |
Human Adherent Cells | SH-SY5Y • IMR32 • LAN5 • A-375 • ARPE-19 • Hela • HeLa S3 • LNCaP • MCF-10A • 293T • MCF-7 • SK-BR3 • OVCAR-3 • Huh7 • GTM-3 • DU145 • HT1080 • DLD-1 • HepG2 • U2OS • HEK293 • U87MG • MIA PaCa-2 • NCI/ ADR-RES |
ON-TARGETplus siRNA Reagents
Trust Thermo Scientific Dharmacon ON-TARGETplus, the best-selling siRNA in the world, to provide the most complete, proven solution for reducing off-target effects that may lead to toxicity or false phenotypes
ON-TARGETplus modifications reduce the overall number of off-targets, and pooling reduces them even further. Off-targets induced by the indicated siRNA reagents targeting 20 human genes were quantified by gene expression microarray analysis (Agilent 22K Platform) and are represented in this boxplot (genes down-regulated by two-fold or more). Each box represents the middle 50% of the data set. Horizontal line in box: Median value of the data set. Vertical bars: Minimum and maximum data values.
siGENOME siRNA Reagents
Recently updated to reflect additions to the RefSeq database, the Thermo Scientific Dharmacon siGENOME collection of pre-designed siRNA are a potent, reliable choice for every gene silencing application
Screening with siGENOME SMARTpool siRNA reagents provides effective target silencing<pEffective silencing achieved for 440 kinase genes when screened with SMARTpool siRNA reagents in two cell lines. Under screening conditions these overall results provide validation of Dharmacon siRNA design and SMARTpool technology for effective target knockdown. HeLa and MCF10a cells were transfected with 50 nM of each siRNA using Thermo Scientific DharmaFECT 1 transfection reagent. Target mRNA levels were determined by branched DNA assay (Panomics Quantigene® Reagent System). The pie chart represents the summary of silencing achieved for the entire data set (880 total data points). The bar graph is a representative sample of genes assayed.
Thermo Scientific Dharmacon miRIDIAN microRNA Reagents
miRIDIAN Mimics
Thermo Scientific Dharmacon miRIDIAN microRNA Mimics are double-stranded RNA oligonucleotides, chemically enhanced with a proprietary design and available for all human, mouse and rat miRNAs in the miRBase Sequence Database</p><p></p>
miRIDIAN microRNA Mimic function for 11 human miRNAs was assayed in HeLa and HepG2 cells 48 hours after transfection of 10 nM mimic using a dual luciferase reporter system and normalized to the control (no mimic).
miRIDIAN Hairpin Inhibitors
Thermo Scientific Dharmacon miRIDIAN microRNA Hairpin Inhibitors are single-stranded, chemically enhanced oligonucleotides available for all human, mouse and rat miRNAs in the miRBase Sequence atabase: http://microrna.sanger.ac.uk
Thermo Scientific Dharmacon Libraries
siRNA Libraries
Thermo Scientific Dharmacon siRNA libraries are available in collections arranged by gene family and function. SMARTpool siRNA reagents are available in 0.5 -2 nmol quantities or Set of Four individual siRNAs. siGENOME siRNA Libraries are also available in Reverse Transfection Format (RTF) plates. A first-of-its-kind format for high-quality results in a fraction of the time
Druggable Genome includes highly relevant targets for superior screening results</p><p>
microRNA Libraries
Thermo Scientific Dharmacon miRIDIAN microRNA Mimic and Hairpin Inhibitor Libraries are complete collections of mimics and inhibitors in 96-well plates which enable classical genetic approaches to
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