연구용제품시약 > Chemical Reagent
[APExBIO한국독점대리점] ARCA Cy5 EGFP mRNA (5-moUTP) "R1009"
바이오클론
APExBIO는 Small molecule, Inhibitor, Peptide 등의
chemical 관련 제품을 직접 생산, 공급하는 브랜드입니다.
바이오클론(주)는
APExBIO의 한국독점대리점이며,
2015년부터 정부의 화학물질의 등록 및 평가 등에
관한 법률(화평법)에 의해 모든 화학물질을 한국환경공단에
신고, 허가 하에 안전하게 수입 공급하고 있습니다.
한국환경공단 신고허가없이 수입되거나 공식대리점이 아닌 무분별한 수입업자에 공급되어진 화학물질품목은 화평법에 의해 문제소지가 발생할수 있습니다 |
Catalog No. R1009
ARCA Cy5 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria.
EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. EGFP mRNA labeled with cyanine 5 can be directly visualized. ARCA Cy5 EGFP mRNA (5-moUTP) is an ideal molecule for determining mRNA delivery and localization and is independent of translation.
Cyanine 5 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 650 nm and 670 nm, respectively. APExBIO ARCA Cy5 EGFP mRNA (5-moUTP) is transcribed in a ratio of 1:3 with Cyanine 5-UTP: 5-methoxy-UTP. Substitution in this ratio results in mRNA that is easy to visualize and can still be translated in cell culture. Translation efficiency is the opposite of Cyanine 5-UTP replacement.
ARCA Cy5 EGFP mRNA (5-moUTP) is restricted using APExBIO's proprietary co-transcriptional capping method to produce a naturally occurring Cap 0 structure with high end capping efficiency.
It is polyadenylation, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics the fully processed mature mRNA.
mRNA Length | 996 nucleotides | ||
Concentration | 1.0 mg/mL | ||
Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
General tips | Dissolve it on ice and take care to prevent RNase contamination degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For the first time, it is gently centrifuged and divided into several parts for stand-alone use. Use RNase-free reagents and consumables, using appropriate RNase-free technology. It can not be added to the serum-containing medium until it is mixed with the transfection reagent. | ||
Shipping Condition | Trial Pack: Dry Ice Transport. |
Homepage : www.bioclone.co.kr
한국공식대리점 바이오클론㈜
T) 02-2690-0058 F) 02-2690-0397
www.bioclone.co.kr
본 정보는 "바이오클론" (으)로 문의 바랍니다.