연구용제품Bio resource > Cell
[IQbioscience한국독점대리점] BALB/c Mouse Peripheral Blood Mononuclear Cells (PBMCs)
바이오클론
2022년 6월 27일로 (주) 바이오클론은 IQBiosciences 사의 한국독점대리점이 되었습니다.
(주) 바이오클론은 고퀄리티 Primary cell (PBMC, Splenocytes, Bone Marrow) 등을 취급하는 IQBiosciences 사의 한국 독점대리점 입니다.
자세한 제품은 아래링크에서 확인하실 수 있습니다 ^^
https://iqbiosciences.com/
SKU: IQB-MPB101
Categories: BALB/c Mouse, Mouse, PBMC
The BALB/c mouse is a commonly-used inbred mouse strain for the study of autoimmune diseases and pathogen-driven immune response. They carry the H-2d haplotype and their immune cells are routinely used in immune-based assays. Based on early studies, the BALB/c mouse is biased towards a Th2 antibody-mediated immune response when challenged appropriately. Thus, the BALB/c mouse is well-studied in the field of immunology and their cells (like these BALB/c Mouse PBMCs) may offer experimental advantages depending on the parameters of your experiments.
BALB/c Mouse PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
https://iqbiosciences.com/product/balbc-mouse-pbmcs/
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