High Quality : Invitrogen 제품 개발자의 USA생산기업 제품
Low Price : 10년동안 소비자 가격 고정
*. 지역 대리점을 통하여 구매하시기 바랍니다.
*. 대리점에 관심있으신 업체분들은 킴바이오로 연락주세요.
LeGene One-Step qRT-PCR Master Mix (2X)
Catalog No. 6470-05: LeGene One-Step qRT-PCR Master Mix (2X), Size: 50 reactions (1.25 ml)
Catalog No. 6470-20: LeGene One-Step qRT-PCR Master Mix (2X), Size: 200 reactions (4X1.25 ml)
Catalog No. 6470-50: LeGene One-Step qRT-PCR Master Mix (2X), Size: 500 reactions (10X1.25 ml)
Store at -20°C
LeGene One-Step qRT-PCR Master Mix is stable for 1 year when stored at -20°C. No significant performance loss was detected after 10 times freeze-thaw cycles. For convenience, it may be stored at 4°C for up to two weeks.
LeGene One-Step qRT-PCR Master Mix is a 2X concentrated formulation mix. It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary 2X qRT-PCR Master Mix contains an engineered RnaUsScript Reverse Transcriptase (RNaseH minus), DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, and stabilizer. LeGene One-Step qRT-PCR Master Mix does not contain an internal reference dye. Please consult our website for the correct product for your real-time PCR system.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 50, 200 or 500 amplification reactions of 50 μl each.