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LeGene qPCR Master Mix, ROX (2X)
Catalog No. 6452-05: LeGene qPCR Master Mix, ROX (2X), Size, 50 reactions (1.25 ml)
Catalog No. 6452-20: LeGene qPCR Master Mix, ROX (2X), Size, 200 reactions (4X1.25 ml)
Catalog No. 6452-50: LeGene qPCR Master Mix, ROX (2X), Size, 500 reactions (10X1.25 ml)
Storage and Stability
LeGene qPCR Master Mix, ROX is stable for 1 year when stored at -20°C. For convenience it may be stored at 4°C for up to 6 months. Repeated freezing and thawing of the mix is not recommended. However, no significant performance loss was detected after 10 times freeze-thaw cycles.
LeGene qPCR Master Mix, ROX is a ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR on systems that require ROX. The proprietary buffer and stabilizers have been optimized to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal. The proprietary 2X qPCR Master Mix, ROX contains DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer, and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from few copies of DNA template, with a broad dynamic range that supports accurate quantification of target sequences. Sufficient reagents are provided for 50, 200 or 500 amplification reactions of 50 μl each.