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LeGene SB-Green qPCR Master Mix (2X)
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LeGene SB-Green qPCR Master Mix (2X)
Catalog No. 6440-05: LeGene SB-Green qPCR Master Mix (2X), Size, 50 reactions (1.25 ml)
Catalog No. 6440-20: LeGene SB-Green qPCR Master Mix (2X), Size, 200 reactions (4X1.25 ml)
Catalog No. 6440-50: LeGene SB-Green qPCR Master Mix (2X), Size, 500 reactions (10X1.25 ml)
Storage and Stability
LeGene SB-Green qPCR Master Mix is stable for 1 year when stored at -20°C, protected from light.
For convenience it may be stored at 4°C for up to 6 months.
Repeated freezing and thawing of the mix is not recommended.
However, no significant performance loss was detected after 10 times freeze-thaw cycles.
Description
LeGene SB-Green qPCR Master Mix is a ready-to-use reaction cocktail that contains all components
except primers and template for real-time quantitative PCR in systems that do not require ROX.
The proprietary buffer and stabilizers have been optimized for SYBR Green qPCR to deliver
maximum PCR efficiency, sensitivity and robust fluorescent signal.
The proprietary 2X qPCR Master Mix contains DnaUs Hot Start Taq DNA polymerase, SYBR Green 1 Dye,
dNTPs, Mg2+, enhancer, and stabilizer.
LeGene qPCR Master Mix does not contain an internal reference dye.
Please consult our website for the correct product for your real-time PCR system.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme
designed to block polymerase activity at ambient temperature.
Once the PCR step reaches denaturation temperature (95oC),
Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from few copies of DNA template,
with a broad dynamic range that supports accurate quantification of target sequences.
Sufficient reagents are provided for 50, 200 or 500 amplification reactions of 50 μl each.
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