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LeGene cDNA Synthesis Master Mix (2X)
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LeGene cDNA Synthesis Master Mix (2X)
Catalog No. 6290-05: LeGene cDNA Synthesis Master Mix (2X), Size: 50 reactions
Catalog No. 6290-20: LeGene cDNA Synthesis Master Mix (2X), Size: 200 reactions
Catalog No. 6290-55: LeGene cDNA Synthesis Master Mix (2X), Size: 1,000 reactions
Store at -20°C
LeGene cDNA Synthesis Master Mix is stable for 1 year when stored at -20°C.
LeGene cDNA Synthesis Master Mix showed no significant loss in functional performance after 20 cycles of freeze-thaw tests.
Description
LeGene cDNA Synthesis Master Mix is a 2X concentrated cDNA synthesis master mix that contains all necessary components
for first strand cDNA synthesis including RnaUs-Script reverse transcriptase, RNase inhibitor, primers, dNTPs, Mg2+, and stabilizers (except RNA template).
This cDNA Synthesis Master Mix provides a sensitive and highly convenient pre-mixed cDNA synthesis solution for two-step RT-PCR.
It uses a combination of RnaUs-Script RT and an optimum blend of random primers and oligo-dT to synthesize high yield cDNA from a wide range of RNA input.
This system can detect mRNA targets up to 8 kb. It is ideally suited for high throughput expression profile studies
using both real-time quantitation and end-point RT-PCR. The system contains all the components needed for first-strand cDNA synthesis.
The reagents are sufficient for 50, 200, or 1,000 cDNA synthesis reactions of 20 µl reaction volume each.
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