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Flow Cytometry Primary Antibodies

Flow cytometry is a core application for most immunologists, so using the best reagents produces the best results. BioLegend has proven our commitment to bringing the highest quality and support to flow cytometry reagents at the best value on the market. We provide an outstanding selection of flow cytometry buffers and fluorophore options for our antibodies, including high performance Brilliant Violet™ conjugates and our featured formats: Spark Dyes and Fire Dyes.

APC/Fire™ 750

	Brightness: 2
	Ex Laser: 633 nm or 640 nm
	Ex Max: 650 nm
	Em Max: 774 nm
	Filter Used: 780/60
	Molecular Weight: 110 kD
	Recommended Application(s): Flow Cytometry

Fluorophore At a Glance:

• More temperature and photo-stable alternative for APC/Cyanine7.
• Improved stability in fixative solutions.
• Lower compensation requirements than APC/Cyanine7 without compromising brightness.
• Minimal non-specific binding to monocytes.
• Like PE/Cyanine7, it is a good fluor to use to detect markers of variable expression because its emission is red-shifted away from neighboring channels.
• APC/Fire™ 750 may exhibit non-specific binding to monocytes and macrophages. Use our True-Stain Monocyte Blocker™ before staining to block this non-specific binding.

View all APC/Fire™ 750 Products.

APC/Fire™ 750 anti-mouse CD68 Antibody

Cat No. : 137041

Clone : FA-11 (See other available formats)

Other Names : Macrosialin

Reactivity : Mouse

Antibody Type : Monoclonal

Host Species : Rat

Application : ICFC - Quality tested

Thioglycollate-elicited BALB/c macrophages were fixed, permeabilized, then stained with anti-mouse F4/80 PerCP and anti-mouse CD68 (clone FA-11) APC/Fire™ 750 (left) or rat IgG2a APC/Fire™ 750 isotype control (right).

APC/Fire 750™ anti-mouse CD44 Antibody

Cat No. : 156003, 156004

Clone : NIM-R8 (See other available formats)

Other Names : Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24

Reactivity : Mouse

Antibody Type : Monoclonal

Host Species : Rat

Application : FC - Quality tested

C57BL/6 mouse splenocytes were stained with anti-mouse CD44 (clone NIM-R8) APC/Fire™ 750 (filled histogram) or rat IgG2a, κ isotype control (open histogram).

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Flow Cytometry Antibodies is Biolegend !!