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[Signagen 한국독점대리점] In Vitro Transfection Reagent for L929 Cells
바이오클론
SIGNAGEN은 저렴한 가격, 높은 효율을 자랑하는 In vitro/In vivo, DNA/siRNA transfection reagent를 판매하는 곳으로, 미국 NIH 연구원들이 직접 설립한 회사입니다.
한국대리점 바이오클론( www.bioclone.co.kr )
Description
GenJet™ DNA In Vitro Transfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death. The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF have been established also.
Refer to the following optimal transfection conditions for maximal transfection efficiency on L929 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: |
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Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
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