연구용제품시약 > Chemical Reagent
[APExBIO]hyPerFUsion™ high-fidelity DNA polymerase
바이오클론
APExBIO는 Small molecule, Inhibitor, Peptide 등의
chemical 관련 제품을 직접 생산, 공급하는 브랜드입니다.
바이오클론(주)는
APExBIO의 한국공식대리점이며,
2015년부터 정부의 화학물질의 등록 및 평가 등에
관한 법률(화평법)에 의해 모든 화학물질을 한국환경공단에
신고, 허가 하에 안전하게 수입 공급하고 있습니다.
한국환경공단 신고허가없이 수입되거나 공식대리점이 아닌 무분별한 수입업자에 공급되어진 화학물질품목은 화평법에 의해 문제소지가 발생할수 있습니다
|
hyPerFUsion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, hyPerFUsion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, hyPerFUsion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. hyPerFUsion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50 fold lower than that of Taq DNA Polymerase and 6 fold lower than that of Pyrococcus furiosus DNA Polymerase (1), hyPerFUsion™ is one of the most accurate thermostable polymerases available. hyPerFUsion™ DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
hyPerFUsion™ DNA Polymerase is supplied with standard 5X hyPerFUsion™ Buffer, which can be used for complex or GC-rich templates. Reactions can also be optimized using the provided DMSO.
1. High fidelity—up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.
2. Enhanced robustness—fewer reaction failures and minimal optimization.
3. Improved yields—high product yields with minimal enzyme amounts (0.5–1 U/50 μL reaction).
4. High speed—increased processivity allows shorter reaction times (10X faster than Pfu).
5. Versatile – Can be used for routine PCR as well as long or difficult (GC-rich) templates.
Homepage : www.bioclone.co.kr
한국공식대리점 바이오클론㈜
T) 02-2690-0058 F) 02-2690-0397
www.bioclone.co.kr
본 정보는 "바이오클론" (으)로 문의 바랍니다.