연구용제품시약 > Kit
[Biorbyt 한국독점대리점] New) TR-FRET ASSAYS Kit
바이오클론
바이오클론은 Birobyt의 한국 독점 대리점으로
합리적인 가격으로 제공하고 있습니다.
** Product Type **
Antibodies (181,605) Small Molecules (24,617)
ELISA Kits (4,376) Biology Tools (52,433)
Proteins (17,660) Services (2)
** Research Area **
Neuroscience & Cancer & Cardiavascular
바이오클론㈜는 Biorbyt의 한국독점대리점이며, 2015년부터 정부의 화학물질 등록 및 평가 등에 관한 법률(화평법)에 의해 모든 화학물질을 한국환경공단에 신고, 허가하에 안전하게 수입, 공급하고 있습니다.
FEATURES
Biorbyt TR-FRET Cellular Phosphorylation Detection Kits are designed for the detection of specific phosphorylated or total (phosphorylated and non-phosphorylated) proteins in cell lysate samples using a simple (add-and-read protocol), homogeneous (no wash steps) TR-FRET assay. These rapid, convenient and sensitive assays monitor pathway activation or inhibition in both adherent and suspension cells. These new assays represent a robust, reliable and rapid alternative to more conventional methods such as Western Blot, ELISA or expensive bead-based assays.
Homogeneous, add-and-read assays; no wash steps
Ready-to-use kit: all required reagents included
More robust, convenient and faster than WB or ELISA
Site and signal pathway-specific
Works with many cell types
Compatible with most TR-FRET compatible plate readers
Cellular kinase assays
Receptor activation studies
Functional testing and screening of small compounds and biotherapeutics
Mechanism of action studies of small compounds and biotherapeutics
For basic research and drug discovery
Europium-labeled antibody
Acceptor-labeled antibody
Lysis buffer
Control cell lysate
Detection buffer
Control cell lysates: can be used in your experiments to ensure kit performance
Cell Lysis Buffers: five available lysis buffers that have been specifically designed to lyse cells under nondenaturing conditions and are fully compatible for TR-FRET applications
TR-FRET compatible Detection Buffer
All assays can be run under a two-plate assay protocol. Cells are plated, treated and then lysed in a 96 well culture plate. Lysates are then transferred to a 96 or 384 assay plate where the TR-FRET reagents are added for direct detection of phosphorylated or total protein. No wash steps are required.
In this protocol suitable for HTS, plating, treating, lysing and detecting are all performed in a single 384 assay plate. No transfer or wash steps are required.
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