한빛사 논문
Moohyun Kim 1,2, Jae Chul Hwang 1,2, Sungjin Min 3, Young-Geun Park 1,2, Suran Kim 3, Enji Kim 1,2, Hunkyu Seo 1,2, Won Gi Chung 1,2, Jakyoung Lee 1,2, Seung-Woo Cho 3,2, Jang-Ung Park 1,2,4
1Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea.
2Center for Nanomedicine, Institute for Basic Science (IBS), Yonsei University, Seoul 03722, Republic of Korea.
3Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
4KIURI Institute, Yonsei University, Seoul 03722, Republic of Korea.
Corresponding Authors: Seung-Woo Cho, Jang-Ung Park
M.K., J.C.H., and S.M. contributed equally to this work.
Abstract
Herein, we present an unconventional method for multimodal characterization of three-dimensional cardiac organoids. This method can monitor and control the mechanophysiological parameters of organoids within a single device. In this method, local pressure distributions of human-induced pluripotent stem-cell-derived cardiac organoids are visualized spatiotemporally by an active-matrix array of pressure-sensitive transistors. This array is integrated with three-dimensional electrodes formed by the high-resolution printing of liquid metal. These liquid-metal electrodes are inserted inside an organoid to form the intraorganoid interface for simultaneous electrophysiological recording and stimulation. The low mechanical modulus and low impedance of the liquid-metal electrodes are compatible with organoids' soft biological tissue, which enables stable electric pacing at low thresholds. In contrast to conventional electrophysiological methods, this measurement of a cardiac organoid's beating pressures enabled simultaneous treatment of electrical therapeutics using a single device without any interference between the pressure signals and electrical pulses from pacing electrodes, even in wet organoid conditions.
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