한빛사 논문
Wookjin Shin1‡, Sumin Jeong1,3‡, Jung-uk Lee1,3, Soo Yeun Jeong1, Jeonghong Shin1,4,5,6, Hyongbum Henry Kim1,4,5,6*, Jinwoo Cheon1,2,3* and Jae-Hyun Lee1,2*
1Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea.
2Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea.
3Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
4Department of Pharmacology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
5Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seou 03722l, Republic of Korea.
6Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
‡W.S. and S.J. contributed equally to this work.
*Corresponding Authors : Hyongbum Henry Kim, Jinwoo Cheon, Jae-Hyun Lee
Abstract
Regulation of genetic activity in single cells and tissues is pivotal to determine key cellular functions in current biomedicine, yet the conventional biochemical activators lack spatiotemporal precision due to the diffusion-mediated slow kinetics and nonselectivity. Here, we describe a magnetogenetic method for target-specific activation of a clustered regularly interspaced short palindromic repeats (CRISPR) system for the regulation of intracellular proteins. We used magnetomechanical force generated by the magnetic nanostructure to activate pre-encoded Piezo1, the mechanosensitive ion channel, on the target cell. The activated Piezo1 further triggers the intracellular Ca2+ signaling pathway, inducing the pre-encoded genes to express genes of interest (GOIs), which is Cas9 protein for the CRISPR regulation of the target proteins. We demonstrated that this magnetogenetic CRISPR system successfully edits the target genome for both in vitro and pseudo-in vivo environments, providing a versatile magnetic platform for remote gene editing of animals with various size scales.
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