한빛사 논문
Joonbum Lee 1, Dong-Hwan Kim 1, Madeline C Karolak 1, Sangsu Shin 1 2, Kichoon Lee 1
1Department of Animal Sciences, The Ohio State University, Columbus, OH 43210.
2Department of Animal Biotechnology, Kyungpook National University, Sangju 37224, South Korea.
To whom correspondence may be addressed : Kichoon Lee
Abstract
Conventional avian genome editing is mediated by isolation, culture, and genome editing of primordial germ cells (PGCs); screening and propagating the genome-edited PGCs; and transplantation of the PGCs into recipient embryos. The PGC-mediated procedures, however, are technically difficult, and therefore, the conventional method has previously been utilized only in chickens. Here, we generated germline mosaic founder chicken and duck lines without the PGC-mediated procedures by injecting an adenovirus containing the CRISPR-Cas9 system into avian blastoderms. Genome-edited chicken and duck offspring produced from the founders carried different insertion or deletion mutations without mutations in the potential off-target sites. Our data demonstrate successful applications of the adenovirus-mediated method for production of genome-edited chicken and duck lines.
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