한빛사 논문
Dongju Shin 1,†, Jungwon Choi 1,†, Ji Hyun Lee2,3,* and Duhee Bang 1,*
1Department of Chemistry, Yonsei University, Seoul, Korea
2Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Korea
3Department of Biomedical Science and Technology, Kyung Hee University, Seoul, Korea
*To whom correspondence should be addressed.
†These authors contributed equally to this work as Co-First Authors.
Abstract
The development of single-cell RNA-seq has broadened the spectrum for biological research by providing a high-resolution analysis of cellular heterogeneity. However, the requirement for sophisticated devices for the compartmentalization of cells has limited its widespread applicability. Here, we develop Onepot-Seq, a device-free method, that harnesses the transient localization of mRNA after lysis to capture single-cell transcriptomes simultaneously in a continuous fluid medium. In mixed-species experiments, we obtained high-quality single-cell profiles. Further, cell type-specific poly(A)-conjugated antibodies allow Onepot-Seq to effectively capture target cells in complex populations. Chemical perturbations to cells can be profiled by Onepot-Seq at single-cell resolution. Onepot-Seq should allow routine transcriptional profiling at single-cell resolution, accelerating clinical and scientific discoveries in many fields of science.
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