한빛사 논문
Xianfeng Zeng,1,4 Xi Xing,1,4 Meera Gupta,2,3,4 Felix C. Keber,2,4 Jaime G. Lopez,4 Ying-Chiang J. Lee,2 Asael Roichman,1,4 Lin Wang,1,4,6 Michael D. Neinast,1,4 Mohamed S. Donia,2 Martin Wuhr,2,4,* Cholsoon Jang,5,* and Joshua D. Rabinowitz1,4,7,8,*
1Department of Chemistry, Princeton University, Princeton, NJ 08544, USA
2Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
3Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
4Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
5Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
6Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, China
7Ludwig Institute for Cancer Research, Princeton Branch, Princeton University, Princeton, NJ 08544, USA
8Lead contact
*Correspondence
Abstract
Great progress has been made in understanding gut microbiomes’ products and their effects on health and disease. Less attention, however, has been given to the inputs that gut bacteria consume. Here, we quantitatively examine inputs and outputs of the mouse gut microbiome, using isotope tracing. The main input to microbial carbohydrate fermentation is dietary fiber and to branched-chain fatty acids and aromatic metabolites is dietary protein. In addition, circulating host lactate, 3-hydroxybutyrate, and urea (but not glucose or amino acids) feed the gut microbiome. To determine the nutrient preferences across bacteria, we traced into genus-specific bacterial protein sequences. We found systematic differences in nutrient use: most genera in the phylum Firmicutes prefer dietary protein, Bacteroides dietary fiber, and Akkermansia circulating host lactate. Such preferences correlate with microbiome composition changes in response to dietary modifications. Thus, diet shapes the microbiome by promoting the growth of bacteria that preferentially use the ingested nutrients.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기