한빛사 논문
Juhyun Kim1,†, Jihye Park1,†, Heejin Kim1, Namil Son1, Eun-Jung Kim1, Jaeil Kim1, Dohwan Byun1, Youngkyung Lee1, Yeong Mi Park1, Divyashree C Nageswaran2, Pallas Kuo2, Teresa Rose3, Tuong Vi T Dang1, Ildoo Hwang1, Christophe Lambing2,3, Ian R Henderson2 & Kyuha Choi1,*
1Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea
2Department of Plant Sciences, University of Cambridge, Cambridge, UK
3Department of Plant Sciences, Rothamsted Research, Harpenden, UK
*Corresponding author.
†These authors contributed equally to this work.
Abstract
The number of meiotic crossovers is tightly controlled and most depend on pro-crossover ZMM proteins, such as the E3 ligase HEI10. Despite the importance of HEI10 dosage for crossover formation, how HEI10 transcription is controlled remains unexplored. In a forward genetic screen using a fluorescent crossover reporter in Arabidopsis thaliana, we identify heat shock factor binding protein (HSBP) as a repressor of HEI10 transcription and crossover numbers. Using genome-wide crossover mapping and cytogenetics, we show that hsbp mutations or meiotic HSBP knockdowns increase ZMM-dependent crossovers toward the telomeres, mirroring the effects of HEI10 overexpression. Through RNA sequencing, DNA methylome, and chromatin immunoprecipitation analysis, we reveal that HSBP is required to repress HEI10 transcription by binding with heat shock factors (HSFs) at the HEI10 promoter and maintaining DNA methylation over the HEI10 5′ untranslated region. Our findings provide insights into how the temperature response regulator HSBP restricts meiotic HEI10 transcription and crossover number by attenuating HSF activity.
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