한빛사 논문
Young Jin Yoo, Joo Hwan Ko, Gil Ju Lee, Jiwon Kang, Min Seok Kim, Stefan G. Stanciu, Hyeon-Ho Jeong, Dae-Hyeong Kim,* and Young Min Song*
Y. J. Yoo, J. H. Ko, J. Kang, M. S. Kim, H.-H. Jeong, Y. M. Song
School of Electrical Engineering and Computer Science Gwangju Institute of Science and Technology Gwangju 61005, Republic of Korea
G. J. Lee
Department of Electronics Engineering Pusan National University Busan 46241, Republic of Korea
S. G. Stanciu
Center for Microscopy-Microanalysis and Information Processing Politehnica University Bucharest Bucharest 060042, Romania
D.-H. Kim
Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826, Republic of Korea
D.-H. Kim
School of Chemical and Biological Engineering Institute of Chemical Processes Seoul National University Seoul 08826, Republic of Korea
D.-H. Kim
Department of Materials Science and Engineering Seoul National University Seoul 08826, Republic of Korea
Y.J.Y. and J.H.K. contributed equally to this work.
*Corresponding author.
Abstract
Bright-field imaging of nanoscale bioparticles is a challenging task for optical microscopy because the light–matter interactions of bioparticles are weak on conventional surfaces due to their low refractive index and small size. Alternatively, advanced imaging techniques, including near-field microscopy and phase microscopy, have enabled visualization and quantification of the bioparticles, but they require assistance of sophisticated/customized systems and post-processing with complex established algorithms. Here, a simple and fast immunoassay device, Gires–Tournois immunoassay platform (GTIP) is presented, which provides unique color dynamics in response to optical environment changes and thus enables the label-free bright-field imaging and facile quantification of bioparticles using conventional optical microscopy. Bioparticles on GTIP slow down the velocity of reflected light, leading to vivid color change according to the local particle density and maximizing chromatic contrast for high spatial distinguishability. The particle distribution and density on the surface of the resonator are readily analyzed through 2D raster-scanning-based chromaticity analysis. GTIP offers multiscale sensing capability for target analytes that possess different refractive indices and sizes.
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