한빛사 논문
Chang Gyun Park, Indong Jun, Sangmin Lee, Chang Seon Ryu, Sang-Ah Lee, Jaeho Park, Hyung-Seop Han, Honghyun Park, Andreas Manz, Heungsoo Shin,* and Young Jun Kim*
C. G. Park, I. Jun, C. S. Ryu, S.-A. Lee, A. Manz, Y. J. Kim
Environmental Safety Group Korea Institute of Science & Technology Europe (KIST-EUROPE) 66123 Saarbrücken, Germany
C. G. Park, A. Manz
Universität des Saarlandes 66123 Saarbrücken, Germany
S. Lee, H. Shin
BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team Hanyang University Seoul 04763, Republic of Korea
S. Lee, H. Shin
Department of Bioengineering Hanyang University Seoul 04763, Republic of Korea
J. Park, H.-S. Han
Center for Biomaterials Biomedical Research Institute Korea Institute of Science and Technology (KIST) Seoul 02792, Republic of Korea
H. Park
Department of Advanced Biomaterials Research Ceramics Materials Division Korea Institute of Materials Science (KIMS) Changwon 51508, Republic of Korea
I.J. and C.G.P. contributed equally to this work.
*Corresponding author.
Abstract
Numerous methods have been introduced to produce 3D cell cultures that can reduce the need for animal experimentation. This study presents a unique 3D culture platform that features bioinspired strands of electrospun nanofibers (BSeNs) and aquatic cell lines to compensate for shortcomings in the current cell spheroid generation techniques. The use of BSeNs in 3D zebrafish liver cell cultures is found to improve liver and reproductive functions through spheroid-based in vitro assays such as whole transcriptome sequencing and reproductive toxicity testing, with optimized properties exhibiting results similar to those obtained for fish embryo acute toxicity (FET, OECD TG 236) following exposure to environmental endocrine-disrupting chemicals (17β-Estradiol (E2), 4-hydroxytamoxifen (4-HT), and bisphenol compounds (bisphenol A (BPA) and 9,9-Bis(4-hydroxyphenyl)fluorene (BPFL)). These findings indicate that the beneficial effects of bioinspired materials that closely mimic ECM environments can yield efficient zebrafish cells with intrinsic functions and xenobiotic metabolism similar to those of zebrafish embryos. As a closer analog for the in vivo conditions that are associated with exposure to potentially hazardous chemicals, the straightforward culture model introduced in this study shows promise as an alternative tool that can be used to further eco-environmental assessment.
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