한빛사 논문
Suran Kim1,7, Sungjin Min1,7, Yi Sun Choi1, Sung-Hyun Jo2, Jae Hun Jung3, Kyusun Han3, Jin Kim1, Soohwan An1, Yong Woo Ji3,4, Yun-Gon Kim2 & Seung-Woo Cho1,5,6,*
1Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea. 2Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of Korea. 3Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul 06229, Republic of Korea. 4Department of Ophthalmology, National Health Insurance Service Ilsan Hospital, Goyang 10444, Republic of Korea. 5Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea. 6Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea. 7These authors contributed equally: Suran Kim, Sungjin Min.
*Corresponding author.
Abstract
Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal tissue-derived extracellular matrix hydrogels are suitable substitutes for Matrigel in gastrointestinal organoid culture. We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels enabled long-term subculture and transplantation of organoids by providing gastrointestinal tissue-mimetic microenvironments. Tissue-specific and age-related extracellular matrix profiles that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that extracellular matrix hydrogels derived from decellularized gastrointestinal tissues are effective alternatives to the current gold standard, Matrigel, and produce organoids suitable for gastrointestinal disease modeling, drug development, and tissue regeneration.
논문정보
관련 링크
연구자 ID
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기