한빛사 논문
Yeon-Woo Cho1†, Seohyeon Jee2†, Intan Rosalina Suhito1, Jeong-Hyeon Lee1, Chun Gwon Park3,4, Kyung Min Choi2,5*, Tae-Hyung Kim1*
1School of Integrative Engineering, Chung-Ang University, 84 Heukseuk-ro, Dongjak-gu, Seoul 06974, Republic of Korea. 2Department of Chemical and Biological Engineering, Sookmyung Women’s University, 100 Cheongpa-ro 47-gil, Yongsan-gu, Seoul 04310, Republic of Korea. 3Department of Biomedical Engineering, SKKU In-stitute for Convergence, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea. 4Department of Intelligent Precision Healthcare Convergence, SKKU Insti-tute for Convergence, Sungkyunkwan University (SKKU) , Suwon, Gyeonggi 16419, Republic of Korea. 5LabInCube Co. Ltd., A304-C2, 45, Yangcheong 4-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, Republic of Korea.
*Corresponding author.
†These authors contributed equally to this work.
Abstract
Stable and continuous supply of essential biomolecules is critical to mimic in vivo microenvironments wherein spontaneous generation of various cell types occurs. Here, we report a new platform that enables highly efficient neuronal cell generation of neural stem cells using single metal-organic framework (MOF) nanoparticle–embedded nanopit arrays (SMENA). By optimizing the physical parameters of homogeneous periodic nanopatterns, each nanopit can confine single nMOFs (UiO-67) that are specifically designed for long-term storage and release of retinoic acid (RA). The SMENA platform successfully inhibited physical interaction with cells, which contributed to remarkable stability of the nMOF (RA⊂UiO-67) structure without inducing nanoparticle-mediated toxicity issues. Owing to the continuous and long-term supply of RA, the neural stem cells showed enhanced mRNA expressions of various neurogenesis-related activities. The developed SMENA platform can be applied to other stem cell sources and differentiation lineages and is therefore useful for various stem cell–based regenerative therapies.
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