한빛사 논문
Sungje Bock1†, Yun‑Sik Choi2†, Minhee Kim1†, Yewon Yun1, Xuan‑Hung Pham1, Jaehi Kim1, Bomi Seong1, Wooyeon Kim1, Ahla Jo1, Kyeong‑Min Ham1, Sung Gun Lee2, Sang Hun Lee3, Homan Kang4, Hak Soo Choi4, Dae Hong Jeong2, Hyejin Chang5*, Dong‑Eun Kim1* and Bong‑Hyun Jun1*
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, South Korea. 2Department of Chemistry Education, Seoul National University, Seoul 08826, South Korea. 3Department of Chemical and Biological Engineering, Hanbat National University, Deajeon 34158, South Korea. 4Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. 5Division of Science Education, Kangwon National University, Chuncheon 24341, South Korea.
*Correspondence
†Sungje Bock, Yun-Sik Choi and Minhee Kim contributed equally to this
work
Abstract
Background
To take advantages, such as multiplex capacity, non-photobleaching property, and high sensitivity, of surface-enhanced Raman scattering (SERS)-based in vivo imaging, development of highly enhanced SERS nanoprobes in near-infrared (NIR) region is needed. A well-controlled morphology and biocompatibility are essential features of NIR SERS nanoprobes. Gold (Au)-assembled nanostructures with controllable nanogaps with highly enhanced SERS signals within multiple hotspots could be a breakthrough.
Results
Au-assembled silica (SiO2) nanoparticles (NPs) (SiO2@Au@Au NPs) as NIR SERS nanoprobes are synthesized using the seed-mediated growth method. SiO2@Au@Au NPs using six different sizes of Au NPs (SiO2@Au@Au50–SiO2@Au@Au500) were prepared by controlling the concentration of Au precursor in the growth step. The nanogaps between Au NPs on the SiO2 surface could be controlled from 4.16 to 0.98 nm by adjusting the concentration of Au precursor (hence increasing Au NP sizes), which resulted in the formation of effective SERS hotspots. SiO2@Au@Au500 NPs with a 0.98-nm gap showed a high SERS enhancement factor of approximately 3.8 × 106 under 785-nm photoexcitation. SiO2@Au@Au500 nanoprobes showed detectable in vivo SERS signals at a concentration of 16 μg/mL in animal tissue specimen at a depth of 7 mm. SiO2@Au@Au500 NPs with 14 different Raman label compounds exhibited distinct SERS signals upon subcutaneous injection into nude mice.
Conclusions
SiO2@Au@Au NPs showed high potential for in vivo applications as multiplex nanoprobes with high SERS sensitivity in the NIR region.
논문정보
관련 링크
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기