한빛사 논문
Minjung Seo1, Yeji Kim2, Byong Duk Ye2, Sang Hyoung Park2, Seog-Young Kim2, Jin Hwa Jung3, Sung Wook Hwang2, Sun Young Chae2, Dong Yun Lee2, Sang Ju Lee2, Seung Jun Oh2, Jihun Kim2, Ji Young Kim4, Sae Jung Na5, Misung Kim6, Sang-Yeob Kim2, Norman Koglin7, Andrew W Stephens8, Mi-Na Kweon2 and Dae Hyuk Moon2
1 Ulsan University Hospital, University of Ulsan College of Medicine;
2 Asan Medical Center, University of Ulsan College of Medicine, Korea, Republic of;
3 Asan Institute for Life Sciences, Asan Medical Center;
4 Hanyang University Medical Center, Hanyang University College of Medicine;
5 Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea;
6 Ulsan University Hospital;
7 Life Molecular Imaging GmbH;
8 Life Molecular Imaging, GmbH
For correspondence or reprints contact: Dae Hyuk Moon
Abstract
We aimed to explore whether the imaging of antiporter system xC– of immune cells with (4S)-4-(3-[18F-fluoropropyl)-L-glutamate (18F-FSPG) positron emission tomography (PET) can assess inflammatory bowel disease (IBD) activity in murine models and patients NCT03546868).
Methods: 18F-FSPG PET imaging was performed to assess IBD activity in mice with dextran sulfate sodium-induced and adoptive T-cell transfer-induced IBD and a cohort of 20 patients at a tertiary care center in South Korea. Immunohistochemical analysis of system xC– and cell surface markers was also studied.
Results: Mice with experimental IBD showed increased intestinal 18F-FSPG uptake and xCT expression in CD11c+, F4/80+, and CD3+ cells in the lamina propria, increases positively associated with clinical and pathological disease activity. 18F-FSPG PET studies in patients, most of whom were clinically in remission or had mildly active IBD, showed that PET imaging was sufficiently accurate in diagnosing endoscopically active IBD and remission in patients and bowel segments. 18F-FSPG PET correctly identified all nine patients with superficial or deep ulcers. Quantitative intestinal 18F-FSPG uptake was strongly associated with endoscopic indices of IBD activity. The number of CD68+xCT+ and CD3+xCT+ cells in 22 bowel segments from patients with ulcerative colitis and the number of CD68+xCT+ cells in seven bowel segments from patients with Crohn’s disease showed a significant positive association with endoscopic indices of IBD activity.
Conclusion: The assessment of system xC– in immune cells may provide diagnostic information on the immune responses responsible for chronic active inflammation in IBD. 18F-FSPG PET imaging of system xC– activity may noninvasively assess the IBD activity.
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