한빛사 논문
Sukjun Kim1,11, Soyoung Kim2,11, Hee Ryung Chang1,11, Doyeon Kim1,11, Junehee Park1, Narae Son1, Joori Park3,4, Minhyuk Yoon2, Gwangung Chae2, Young-Kook Kim5, V. Narry Kim1,6, Yoon Ki Kim3,4, Jin-Wu Nam7, Chanseok Shin2,8,9,* & Daehyun Baek1,10,*
1School of Biological Sciences, Seoul National University, Seoul, Republic of Korea. 2Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea. 3Creative Research Initiatives Center for Molecular Biology of Translation, Korea University, Seoul, Republic of Korea. 4Division of Life Sciences, Korea University, Seoul, Republic of Korea. 5Department of Biochemistry, Chonnam National University Medical School, Hwasun, Jeollanamdo, Republic of Korea. 6Center for RNA Research, Institute for Basic Science, Seoul, Republic of Korea. 7Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Republic of Korea. 8Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, Republic of Korea. 9Research Center for Plant Plasticity, Seoul National University, Seoul, Republic of Korea. 10Bioinformatics Institute, Seoul National University, Seoul, Republic of Korea.
11These authors contributed equally: Sukjun Kim, Soyoung Kim, Hee Ryung Chang, Doyeon Kim.
*Corresponding author.
Abstract
Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among the currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful of RBPs known to enhance MT and several others reported to suppress MT, leaving the global impact of RBPs on MT elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 human RBPs and evaluated the quantitative effect of individual RBPs on MT efficacy. In contrast to previous studies, we show that most RBPs significantly affect MT and that all of those MT-regulating RBPs function as MT enhancers rather than suppressors, by making the local secondary structure of the target site accessible to Argonaute. Our findings illuminate the unappreciated regulatory impact of human RBPs on MT, and as these RBPs may play key roles in the gene regulatory network governed by metazoan miRNAs, MT should be understood in the context of co-regulating RBPs.
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