한빛사 논문
Ji Hoon Ahn,1,2 JungMo Kim,1 Seon Pyo Hong,1 Sung Yong Choi,3 Myung Jin Yang,1,2 Young Seok Ju,2 Young Tae Kim,4 Ho Min Kim,2,5 MD Tazikur Rahman,6,7 Man Ki Chung,8 Sang Duk Hong,8 Hosung Bae,1,* Chang-Seop Lee,7,9,* and Gou Young Koh1,2,*
1Center for Vascular Research, Institute for Basic Science (IBS), Daejeon, Republic of Korea. 2Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. 3Department of Otorhinolaryngology – Head and Neck Surgery, Uijeongbu Eulji Medical Center, Eulji University School of Medicine, Daejeon, Republic of Korea. 4Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul, Republic of Korea. 5Center for Biomolecular and Cellular Structure, IBS, Daejeon, Republic of Korea. 6Department of Medical Science, Jeonbuk National University Medical School, Jeonju, Republic of Korea. 7Research Institute of Clinical Medicine of Jeonbuk National University – Biomedical Research Institute of Jeonbuk, National University Hospital, Jeonju, Republic of Korea. 8Department of Otorhinolaryngology – Head and Neck Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. 9Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju, Republic of Korea.
Authorship note: JHA and JK contributed equally to this work.
*Address correspondence to: Gou Young Koh, Center for Vascular Research, IBS; 291 Daehak-ro, Daejeon 34131, Republic of Korea.
Or to: ChangSeop Lee, Department of Internal Medicine, Jeonbuk National University Medical School; 20 Geonji-ro, Jeonju 54896, Republic of Korea. Or to: Hosung Bae, Center for Vascular Research, IBS; 291 Daehak-ro, Daejeon 34131, Republic of Korea.
Abstract
The upper respiratory tract is compromised in the early period of COVID-19, but SARS-CoV-2 tropism at the cellular level is not fully defined. Unlike recent single-cell RNA-Seq analyses indicating uniformly low mRNA expression of SARS-CoV-2 entry–related host molecules in all nasal epithelial cells, we show that the protein levels are relatively high and that their localizations are restricted to the apical side of multiciliated epithelial cells. In addition, we provide evidence in patients with COVID-19 that SARS-CoV-2 is massively detected and replicated within the multiciliated cells. We observed these findings during the early stage of COVID-19, when infected ciliated cells were rapidly replaced by differentiating precursor cells. Moreover, our analyses revealed that SARS-CoV-2 cellular tropism was restricted to the nasal ciliated versus oral squamous epithelium. These results imply that targeting ciliated cells of the nasal epithelium during the early stage of COVID-19 could be an ideal strategy to prevent SARS-CoV-2 propagation.
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