한빛사 논문
연세대학교
Juyoung Kwon†,§, Jong Seung Lee‡,§, Jaejun Lee†, Jukwan Na†, Jaesuk Sung†, Hyo-Jung Lee†, Hankyul Kwak‡, Eunji Cheong‡, Seung-Woo Cho‡,||, #,*, and Heon-Jin Choi†,*
†Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea
‡Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
||Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea
#Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea
J.K. and J.S.L. contributed equally to this work.
*Corresponding authors Prof. Seung-Woo Cho, Prof. Heon-Jin Choi
Abstract
Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기