한빛사 논문
Hye Ryoung Heo1,2, Kye Il Joo2, Jeong Hyun Seo3, Chang Sup Kim4,* & Hyung Joon Cha2,*
1School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea.
2Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Republic of Korea.
3School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea. 4School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.
*Corresponding author
Abstract
On-chip glycan biosynthesis is an effective strategy for preparing useful complex glycan sources and for preparing glycan-involved applications simultaneously. However, current methods have some limitations when analyzing biosynthesized glycans and optimizing enzymatic reactions, which could result in undefined glycan structures on a surface, leading to unequal and unreliable results. In this work, a glycan chip is developed by introducing a pH-responsive i-motif DNA linker to control the immobilization and isolation of glycans on chip surfaces in a pH-dependent manner. On-chip enzymatic glycosylations are optimized for uniform biosynthesis of cancer-associated Globo H hexasaccharide and its related complex glycans through stepwise quantitative analyses of isolated products from the surface. Successful interaction analyses of the anti-Globo H antibody and MCF-7 breast cancer cells with on-chip biosynthesized Globo H-related glycans demonstrate the feasibility of the structure-switchable DNA linker-based glycan chip platform for on-chip complex glycan biosynthesis and glycan-involved applications.
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