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Abstract
Seon-Min Oh, Cheon-Seok Park, Young-Rok Kim, Moo-Yeol Baik*
Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University
*Corresponding author
Abstract
Short chain glucan aggregates (SCGAs) forming ability of starches from different botanical sources and physicochemical characteristics of the produced SCGAs from various starches were investigated. SCGAs were obtained by enzymatic hydrolysis and centrifugation followed by self-assembly of their hydrolysates in supernatant. Size of SCGAs was 0.68-1.30 μm and its shape was spherical with high agglomeration. Yield showed negative correlation with amylose content (R2 = 0.9024) indicating that starches with low amylose content or high amylopectin content produced more SCGAs. Chain length distribution of SCGAs prepared from various starches were similar possibly due to the removal of large and insoluble portions using centrifugation after enzymatic hydrolysis. Main constituent of SCGAs was a B1 chain (13≤DP≤24). All SCGAs showed B-type crystal pattern with relative crystallinity of 14.5-19.4%. DSC endothermic transition of SCGAs occurred at higher temperature than native starch indicating the improved thermal stability. Colloidal dispersion stability of SCGAs was increased with increasing absolute value of zeta potential and decreasing particle size. Consequently, SCGAs can be obtained from all kinds of starches regardless of amylose content and type of crystallinity, and have distinctive physicochemical properties. This study provides helpful information on the preparation of SCGA from various starches and their characteristics resulting that SCGAs can be customized with appropriate properties using different starches.
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