한빛사 논문
Mingzhe Shena,b,c, Chae Jin Limc,d, Junghoon Parkc,d, Jeong Eun Kimc, Dongwon Baekc, Jaesung Name, Sang Yeol Leec, Jose M. Pardof,g, Woe-Yeon Kimc, David Mackeya,b,1, and Dae-Jin Yund,1
aDepartment of Horticulture and Crop Science, The Ohio State University, Columbus, OH 43210; bDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210; cDivision of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Institute of Agriculture and Life Science, Gyeongsang National University, 52828 Jinju, Republic of Korea; dDepartment of Biomedical Science and Engineering, Konkuk University, 05029 Seoul, South Korea; eDepartment of Molecular Biotechnology, Dong-A University, 604-714 Busan, South Korea; fInstitute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas, 41092 Seville, Spain; and gInstitute of Plant Biochemistry and Photosynthesis, University of Seville, 41092 Seville, Spain
1To whom correspondence may be addressed.
Abstract
Transcriptional regulation is a complex and pivotal process in living cells. HOS15 is a transcriptional corepressor. Although transcriptional repressors generally have been associated with inactive genes, increasing evidence indicates that, through poorly understood mechanisms, transcriptional corepressors also associate with actively transcribed genes. Here, we show that HOS15 is the substrate receptor for an SCF/CUL1 E3 ubiquitin ligase complex (SCFHOS15) that negatively regulates plant immunity by destabilizing transcriptional activation complexes containing NPR1 and associated transcriptional activators. In unchallenged conditions, HOS15 continuously eliminates NPR1 to prevent inappropriate defense gene expression. Upon defense activation, HOS15 preferentially associates with phosphorylated NPR1 to stimulate rapid degradation of transcriptionally active NPR1 and thus limit the extent of defense gene expression. Our findings indicate that HOS15-mediated ubiquitination and elimination of NPR1 produce effects contrary to those of CUL3-containing ubiquitin ligase that coactivate defense gene expression. Thus, HOS15 plays a key role in the dynamic regulation of pre- and postactivation host defense.
HOS15, NPR1, ubiquitin ligase, corepressor, plant immunity
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