한빛사 논문
Amir Roshanzadeh1,§, Sangwoo Park2,§, Sarina Ehteshamzadeh Ganjbakhsh1, Jeongwon Park2, Dong-Hyun Lee3, Seongsoo Lee2*, Eung-Sam Kim1,3,4,*
1School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea.
2Gwangju Center, Korea Basic Science Institute (KBSI), Gwangju, 61186, Republic of Korea.
3Department of Biological Sciences and Research Center of Ecomimetics, Chonnam National University, Gwangju, 61186, Republic of Korea.
4Center for Next Generation Sensor Research and Development, Chonnam National University, Gwangju, 61186, Republic of Korea.
*Corresponding Authors: Seongsoo Lee, Ph.D., Eung-Sam Kim, Ph.D.
§Authors contributed equally
Abstract
Polystyrene nanoparticles (PS-NPs) derived from both environmental and occupational sources are an important class of ultrafine particles associated with human pulmonary disorders. The effects of surface charges of particle internalization and toxicity to alveolar cells, especially under conditions comparable to those found during breathing, have not been examined. Here, we applied cyclic stretches (CS) to human alveolar cells during nanoparticle exposure and show an enhanced accumulation of positively charged polystyrene nanoparticles as compared to similar negatively charged particles. The cellular uptake of the positive particles into live cells was visualized with three-dimensional optical diffraction tomography (3-D ODT). The simultaneous application of both periodic stretching as well as positively charged nanoparticles led to blebbing morphology and activation of apoptotic signaling compared to control cells. Our findings provide a better understanding of how surface charge mediates the uptake and toxicity of nanoplastics under the dynamical mechanical conditions relevant for breathing exposures.
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TOP52020년 후보
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