한빛사 논문
Bumsik Cho1,7, Sang-Ho Yoon1,7, Daewon Lee1, Ferdinand Koranteng1, Sudhir Gopal Tattikota2, Nuri Cha1, Mingyu Shin1, Hobin Do1, Yanhui Hu2, Sue Young Oh3, Daehan Lee1, A. Vipin Menon1, Seok Jun Moon3, Norbert Perrimon2,4, Jin-Wu Nam1,5,6,* & Jiwon Shim1,5,6,*
1Department of Life Sciences, College of Natural Science, Hanyang University, Seoul 04736, Republic of Korea.
2Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
3Department of Oral Biology, Yonsei University, College of Dentistry, Seoul 03722, Republic of Korea.
4Howard Hughes Medical Institute, Boston, MA 02115, USA.
5Research Institute for Natural Sciences, Hanyang University, Seoul 04736, Republic of Korea.
6Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04736, Republic of Korea.
7These authors contributed equally: Bumsik Cho, SangHo Yoon.
*Corresponding author
Abstract
The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.
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