한빛사 논문
Sunhong Mina, Yoo Sang Jeonb, Hee Joon Jungc,d,e, Chandra Khatuaa, Na Lif, Gunhyu Baea, Hyojun Choia, Hyunsik Honga, Jeong Eun Shina, Min Jun Koa, Han Seok Koa, Indong Junf, Hong En Fua, Seung Hyun Kima, Ramar Thangama, Jae‐Jun Songf, Vinayak P. Dravidc,d,e, Young Keun Kima,g,*, Heemin Kanga,g,*
aDepartment of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea
bResearch Institute of Engineering and Technology, Korea University, Seoul 02841, Republic of Korea
cDepartment of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA
dInternational Institute for Nanotechnology, Evanston, IL 60208, USA
eNUANCE Center, Northwestern University, Evanston, IL 60208, USA
fDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul 08308, Republic of Korea
gDepartment of Biomicrosystem Technology, Korea University, Seoul 02841, Republic of Korea
S.M., Y.J., and H.J. contributed equally to this work.
*To whom correspondence should be addressed.
Abstract
The native extracellular matrix (ECM) can exhibit heterogeneous nano‐sequences periodically displaying ligands to regulate complex cell–material interactions in vivo. Herein, an ECM‐emulating heterogeneous barcoding system, including ligand‐bearing Au and ligand‐free Fe nano‐segments, is developed to independently present tunable frequency and sequences in nano‐segments of cell‐adhesive RGD ligand. Specifically, similar exposed surface areas of total Fe and Au nano‐segments are designed. Fe segments are used for substrate coupling of nanobarcodes and as ligand‐free nano‐segments and Au segments for ligand coating while maintaining both nanoscale (local) and macroscale (total) ligand density constant in all groups. Low nano‐ligand frequency in the same sequences and terminally sequenced nano‐ligands at the same frequency independently facilitate focal adhesion and mechanosensing of stem cells, which are collectively effective both in vitro and in vivo, thereby inducing stem cell differentiation. The Fe/RGD‐Au nanobarcode implants exhibit high stability and no local and systemic toxicity in various tissues and organs in vivo. This work sheds novel insight into designing biomaterials with heterogeneous nano‐ligand sequences at terminal sides and/or low frequency to facilitate cellular adhesion. Tuning the electrodeposition conditions can allow synthesis of unlimited combinations of ligand nano‐sequences and frequencies, magnetic elements, and bioactive ligands to remotely regulate numerous host cells in vivo.
Keywords : cell adhesion, nano-ligand frequencies, nano-ligand sequences, stem cell differentiation, unlimited nanobarcode tunability
논문정보
TOP52020년 후보
관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기