한빛사 논문
Sungryong Oh1,†, Kyungjin Boo1,†, Jaebeom Kim1, Seon Ah Baek1, Yoon Jeon2, Junghyun You3, Ho Lee2, Hee-Jung Choi3, Daechan Park4,*, Ji Min Lee5,* and Sung Hee Baek1,*
1Creative Research Initiatives Center for Epigenetic Code and Diseases, Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea, 2Graduate School of Cancer Science and Policy, Research Institute, National Cancer Center, Goyang 10408, South Korea, 3Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea, 4Department of Biological Sciences, College of Natural Sciences, Ajou University, Suwon 16499, South Korea and 5Department of Molecular Bioscience, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, South Korea
†The authors wish it to be known that, in their opinion, the first two authors should be regarded as Joint First Authors.
*Corresponding author
Abstract
Epigenetic regulation is important for establishing lineage-specific gene expression during early development. Although signaling pathways have been well-studied for regulation of trophectoderm reprogramming, epigenetic regulation of trophectodermal genes with histone modification dynamics have been poorly understood. Here, we identify that plant homeodomain finger protein 6 (PHF6) is a key epigenetic regulator for activation of trophectodermal genes using RNA-sequencing and ChIP assays. PHF6 acts as an E3 ubiquitin ligase for ubiquitination of H2BK120 (H2BK120ub) via its extended plant homeodomain 1 (PHD1), while the extended PHD2 of PHF6 recognizes acetylation of H2BK12 (H2BK12Ac). Intriguingly, the recognition of H2BK12Ac by PHF6 is important for exerting its E3 ubiquitin ligase activity for H2BK120ub. Together, our data provide evidence that PHF6 is crucial for epigenetic regulation of trophectodermal gene expression by linking H2BK12Ac to H2BK120ub modification.
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