한빛사 논문
Byung-Hyun Cha, Jin-Su Kim, Alvin Bello, Geun-Hui Lee, Do-Hyun Kim, Byoung Ju Kim, Yoshie Arai, Bogyu Choi, Hansoo Park, and Soo-Hong Lee*
Dr. B.-H. Cha
Division of Cardio-Thoracic Surgery, Department of Surgery, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Dr. J.-S. Kim
CellenGene R&D Center, Open Innovation Building, Seoul 02455, Republic of Korea
Dr. J.-S. Kim, G.-H. Lee, Dr. B. Choi
Department of Biomedical Science, CHA University, CHA Biocomplex Seongnam-si, Gyeonggi-do 13488, Republic of Korea
A. Bello, Prof. H. Park
Department of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
D.-H. Kim, Dr. B. J. Kim, Dr. Y. Arai, Prof. S.-H. Lee
Department of Medical Biotechnology, Dongguk University, 32 Dongguk-ro, Ilsandong-gu Goyang, Gyeonggi 10326, Republic of Korea
*Corresponding author
Abstract
Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin α 5β 1 (fibronectin (FN) receptor)‐to‐FN interactions (hESC‐FN‐MSCs) is successfully developed. It is demonstrated that hESC‐FN‐MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC‐FN‐MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine.
논문정보
관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기